Overview

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Overview

This dashboard is for HDX data for BF2-B15:01 and B2-B19:01 +/- peptide

  • This tab contains the mean uptake per timepoint overlaid onto the structures as animations.

  • The second tab has heatmaps comparing peptide free to peptide bound for each allotype, providing a measure of peptide dependent exchange.

  • The third and fourth tabs compare allotypes peptide free and peptide bound to give a measure of allotype dependent exchange using the mean HDX across overlapping peptides for each timepoint.

  • The fifth and six tabs show the HDX maps of the individual peptides. You may need to zoom in to see the peptides clearly.

  • The seventh tab shows HDX exchange rates for selected fragments using the mean of the mean uptake for that region as a percentage of maximum theoretical exhange.

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BF2*15:01 Heavy Chain + peptide

BF2*15:01 Heavy Chain - peptide

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BF2*19:01 Heavy Chain + peptide

BF2*19:01 Heavy Chain - peptide

Peptide dependent exchange

HDX exchange values for Peptide bound vs. Peptide free. Negative values (red) represent % greater exchange in the peptide free, 0 no difference (blue), and positive values (white) represent % less exchange in the peptide free than peptide bound.

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BF2*15:01 Heavy Chain +/- peptide

BF2*15:01 B2m +/- peptide

Column

BF2*19:01 Heavy Chain +/- peptide

BF2*19:01 B2m +/- peptide

Allotype dependent HC exchange

HDX exchange values +/- peptide as a percentage of maximum theoretical exhange.

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BF2*15:01 Heavy chain + peptide

BF2*15:01 Heavy chain - peptide

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BF2*19:01 Heavy chain + peptide

BF2*19:01 Heavy chain - peptide

Allotype dependent \(\beta_{2}\)m exchange

HDX exchange values +/- peptide as a percentage of maximum theoretical exhange.

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BF2*15:01 \(\beta_{2}\)m + peptide

BF2*15:01 \(\beta_{2}\)m - peptide

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BF2*19:01 \(\beta_{2}\)m + peptide

BF2*19:01 \(\beta_{2}\) - peptide

HC peptide maps

HDX exchange values +/- peptide as a percentage of maximum theoretical exhange.

Column

BF2*15:01 Heavy chain + peptide

BF2*15:01 Heavy chain - peptide

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BF2*19:01 Heavy chain + peptide

BF2*19:01 Heavy chain - peptide

\(\beta_{2}\)m peptide maps

HDX exchange values +/- peptide as a percentage of maximum theoretical exhange.

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BF2*15:01 \(\beta_{2}\)m + peptide

BF2*15:01 \(\beta_{2}\)m - peptide

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BF2*19:01 \(\beta_{2}\)m + peptide

BF2*19:01 \(\beta_{2}\)m - peptide

Fragment uptake rates

HDX exchange rates for selected fragments using the mean of the mean uptake for that region as a percentage of maximum theoretical exhange.

Column

\(\alpha_{1}\) peptide

\(\alpha_{2}\) peptide

\(\alpha_{3}\) peptide

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\(\alpha_{2}\) peptide

Heavy chain linker

\(\alpha_{3}\) peptide

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\(\beta_{2}\)m peptide

\(\beta_{2}\)m peptide

\(\beta_{2}\)m peptide